2EYQ
Crystal structure of Escherichia coli transcription-repair coupling factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2004-07-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98166 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.870, 161.990, 161.730 |
| Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.29500 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 61409 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 28% pentaerythritol ethoxylate 100 mM HEPES, pH 7.5 75mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
