2EXL
GRP94 N-terminal Domain bound to geldanamycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 102 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-05 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.820, 84.360, 95.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.610 - 2.350 |
| Rwork | 0.221 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yt1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22646 | |
| <I/σ(I)> | 12.06 | 3.55 |
| Completeness [%] | 93.2 | 46.5 |
| Redundancy | 3.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 34% PEG400, 60 mM MgCl2, Tris pH 7.6, Geldanamycin delivered as 5uL to 50uL of protein Stock Geldanamycin is 50mM, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
