2EWA

Dual binding mode of pyridinylimidazole to MAP kinase p38

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	66.192, 74.650, 78.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.241
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.300	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.391
Number of reflections	23329
<I/σ(I)>	8.2	2
Completeness [%]	99.5	99.3
Redundancy	4.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1