2ESA
GRP94 n-terminal domain bound to geldanamycin: effects of mutants 168-169 KS-AA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 102 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.330, 66.390, 75.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.160 - 1.900 |
| Rwork | 0.180 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yt1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 19422 | |
| <I/σ(I)> | 13.75 | 5.55 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.336 | 4.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 38% PEG 550MME, 30mM MgCl2, 100mM Tris, 3.7mM Geldanamycin delivered as 4uL to 50uL of protein. Stock Geldanamycin is 50mM in DMSO., pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
