2EHH
Crystal structure of dihydrodipicolinate synthase from aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-11-20 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.321, 146.953, 97.877 |
Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
Resolution | 19.760 - 1.900 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o5k |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CCP4 ((MOLREP)) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.091 | 0.465 |
Number of reflections | 126980 | |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 5.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 8 | 291 | 0.4M NaH2PO4, 1.6M K2HPO4, imidazole, 0.2M NaCl, pH 8.0, LIQUID DIFFUSION, temperature 291K |