2EFC
Ara7-GDP/AtVps9a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 58.377, 58.474, 68.714 |
Unit cell angles | 81.68, 86.89, 72.97 |
Refinement procedure
Resolution | 68.040 - 2.090 |
R-factor | 0.22409 |
Rwork | 0.221 |
R-free | 0.27419 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | nucleotide-free Ara7/AtVps9a which was solved by MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.263 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.072 | 0.295 |
Number of reflections | 48167 | |
<I/σ(I)> | 10.6 | 2.3 |
Completeness [%] | 95.1 | 78.9 |
Redundancy | 2.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG3350, 200mM lithium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |