2EEP
Prolyl Tripeptidyl Aminopeptidase Complexed with an Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 150.103, 150.103, 160.985 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23100 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2d5l |
RMSD bond length | 0.019 |
RMSD bond angle | 1.617 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | |
Number of reflections | 54747 | |
<I/σ(I)> | 79.2 | 14 |
Completeness [%] | 99.9 | 100 |
Redundancy | 20.7 | 19.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 1.1M Potassium Sodium tartrate, 0.2M Lithium Sulfate, 0.1M CHES Buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |