2E9W
Crystal structure of the extracellular domain of Kit in complex with stem cell factor (SCF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.249999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 269.477, 52.072, 189.791 |
| Unit cell angles | 90.00, 108.00, 90.00 |
Refinement procedure
| Resolution | 38.960 - 3.500 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EXZ and 2EC8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.061 | 0.176 |
| Number of reflections | 31962 | |
| <I/σ(I)> | 14.9 | 6.6 |
| Completeness [%] | 98.5 | 91.4 |
| Redundancy | 3.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 8-12% PEG 8000, 5-8% ethylene glycol, 0.2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, temperature 277K |
