2E31

Structural basis for selection of glycosylated substrate by SCFFbs1 ubiquitin ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-09
Detector	Bruker DIP-6040
Wavelength(s)	0.9000
Spacegroup name	P 32 2 1
Unit cell lengths	106.408, 106.408, 109.854
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.220 - 2.400
R-factor	0.23605
Rwork	0.233
R-free	0.29136
Structure solution method	SIR-AS, MOLECULAR REPLACEMENT
Starting model (for MR)	1LDK and 1UMH
RMSD bond length	0.010
RMSD bond angle	1.351
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.800	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.045	0.327
Number of reflections	27964
<I/σ(I)>	18.1	2.7
Completeness [%]	97.7	93
Redundancy	5.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	2.0M ammonium sulphate, 0.1M sodium citrate, 30mM chitobiose, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K