2E0M
Mutant Human Ribonuclease 1 (T24L, Q28L, R31L, R32L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 93.510, 93.510, 94.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.19527 |
| Rwork | 0.194 |
| R-free | 0.21364 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7rsa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.085 | 0.446 |
| Number of reflections | 44558 | |
| <I/σ(I)> | 34.6 | 6.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.2 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 293 | 30% PEG 400, 0.1M cadmium chloride, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, temperature 293K |
