2DSU

Binding of chitin-like polysaccharide to protective signalling factor: Crystal structure of the complex formed between signalling protein from sheep (SPS-40) with a tetrasaccharide at 2.2 A resolution

Replaces:  2AO8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2005-07-20
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.750, 66.510, 107.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.000 - 2.200
R-factor	0.187
Rwork	0.187
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dpe
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	AUTOMAR
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	23000
<I/σ(I)>	14.7	2.6
Completeness [%]	95.8	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	25MM TRIS-HCL, 50MM NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K