2DSR
Structural Basis for the Inhibition of Insulin-like Growth Factors by IGF Binding Proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-06-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.400, 50.250, 64.300 |
Unit cell angles | 90.00, 115.30, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.20173 |
Rwork | 0.199 |
R-free | 0.25641 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dsq |
RMSD bond length | 0.007 |
RMSD bond angle | 1.085 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 12370 | |
Completeness [%] | 94.0 | 68.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 1M lithium sulfate monohydrate, 2% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 290K |