2DBY
Crystal structure of the GTP-binding protein YchF in complexed with GDP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-07 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 0.9794, 0.9789, 0.9640 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.384, 133.169, 46.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.730 - 1.760 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 37754 | |
| <I/σ(I)> | 23.9059 | 4.25293 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.6638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 300 | 1.7M sodium formate, 0.1M sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
