2DBQ
Crystal Structure of Glyoxylate Reductase (PH0597) from Pyrococcus horikoshii OT3, Complexed with NADP (I41)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-27 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97892 |
Spacegroup name | I 41 |
Unit cell lengths | 114.252, 114.252, 119.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.290 - 1.700 |
Rwork | 0.131 |
R-free | 0.14290 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.392 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 84158 | |
<I/σ(I)> | 21.94 | 4.697 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |