2D4K
Monoclinic hen egg-white lysozyme crystallized at 313K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2002-05-29 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.217, 63.484, 59.189 |
| Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
| Resolution | 19.700 - 1.150 |
| R-factor | 0.133 |
| Rwork | 0.132 |
| R-free | 0.16400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lys |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.032 |
| Data reduction software | SaintPlus |
| Data scaling software | SAINTPLUS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.119 | 0.336 |
| Number of reflections | 67029 | |
| Completeness [%] | 93.9 | 62.2 |
| Redundancy | 4.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch method | 7.6 | 313 | 2% protein, 10% sodium chloride, 5% 1-propanol, pH 7.6, batch method, temperature 313K |
