2D2I
Crystal Structure of NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase from Synechococcus Sp. complexed with Nadp+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.006, 79.588, 206.155 |
| Unit cell angles | 90.00, 101.31, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.500 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 | 0.265 |
| Number of reflections | 71057 | |
| <I/σ(I)> | 7.7 | 2.8 |
| Completeness [%] | 85.8 | 85.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | ammonium sulfate, potassium sodium tartrate, citrate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
