2CXE

Crystal structure of octameric ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Pyrococcus horikoshii OT3 (form-2 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-17
Detector	ADSC QUANTUM
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	171.478, 149.207, 107.461
Unit cell angles	90.00, 127.37, 90.00

Refinement procedure

Resolution	46.720 - 3.000
R-factor	0.295
Rwork	0.295
R-free	0.32800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.074
RMSD bond angle	5.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	39367

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	magnesium chloride, sodium chloride, sodium acetate, PEG6000, Tris, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 293K