2CWA

Crystal Structure Of The Single-stranded DNA Binding Protein From Thermus Thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.90000, 0.97905, 0.97940
Spacegroup name	P 65 2 2
Unit cell lengths	91.560, 91.560, 132.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.650 - 1.960
R-factor	0.242
Rwork	0.242
R-free	0.28200
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.650	2.030
High resolution limit [Å]	1.960	1.960
Rmerge	0.058	0.319
Number of reflections	24054
<I/σ(I)>	24.3	7.2
Completeness [%]	99.2	96
Redundancy	18.99	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	293	Sodium Nitrate, Tris-HCl, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K