2CW0

Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	110
Detector technology	CCD
Collection date	2003-10-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 32
Unit cell lengths	236.150, 236.150, 249.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.300
Rwork	0.282
R-free	0.32000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iw7
RMSD bond length	0.009
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.138
Number of reflections	115975
<I/σ(I)>	8.16
Completeness [%]	98.5	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.85	293	33mM magnesium formate over a reservoir of 33mM magnesium formate, 30mM sodium citrate, pH 5.85, VAPOR DIFFUSION, HANGING DROP, temperature 293K