2CMT
The structure of reduced cyclophilin A from s. mansoni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2006-04-24 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.654, 59.213, 61.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.800 - 1.500 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zmf |
RMSD bond length | 0.005 |
RMSD bond angle | 0.985 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.020 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.170 |
Number of reflections | 23994 | |
<I/σ(I)> | 7.21 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.6 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.1M IMIDAZOLE/MALATE PH 5.5, 20 % PEG 600 |