2CFM
ATP-DEPENDENT DNA LIGASE FROM PYROCOCCUS FURIOSUS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-21 |
| Detector | RIGAKU RAXIS-IV |
| Wavelength(s) | 0.9797, 0.9791, 0.9787, 0.9587 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.866, 87.399, 62.648 |
| Unit cell angles | 90.00, 109.22, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23100 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.390 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 56984 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.4 | 98.5 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ETHANOL, PH 8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
