2C7W
Crystal Structure of human vascular endothelial growth factor-B: Identification of amino acids important for angiogeninc activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-18 |
Detector | ADSC CCD |
Spacegroup name | P 64 |
Unit cell lengths | 120.806, 120.806, 39.826 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.480 |
R-factor | 0.286 |
Rwork | 0.286 |
R-free | 0.31000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vpf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.096 | 0.630 |
Number of reflections | 12054 | |
<I/σ(I)> | 12.9 | 2.4 |
Completeness [%] | 97.4 | 99.3 |
Redundancy | 9.1 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 0.1M HEPES (PH 6.8), 0.5M AMMONIUM SULPHATE 50%, MPD |