2C6Q

Crystal structure of human guanosine monophosphate reductase 2 GMPR2 in complex with IMP and NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-30
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	131.676, 141.366, 164.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.165
Rwork	0.163
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bzn
RMSD bond length	0.019
RMSD bond angle	1.760
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.090	0.510
Number of reflections	332307
<I/σ(I)>	14.5	3.9
Completeness [%]	99.3	99.7
Redundancy	6.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1