2C46
CRYSTAL STRUCTURE OF THE HUMAN RNA guanylyltransferase and 5'- phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.400, 161.600, 60.700 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 80.850 - 1.600 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9s |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 133485 | |
| <I/σ(I)> | 10.29 | 2.71 |
| Completeness [%] | 90.4 | 87.9 |
| Redundancy | 3.79 | 2.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M POTASSIUM CITRATE |
