2C36
Structure of unliganded HSV gD reveals a mechanism for receptor- mediated activation of virus entry
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-12 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.252, 106.193, 130.315 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.650 - 2.110 |
R-factor | 0.197 |
Rwork | 0.194 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l2g |
RMSD bond length | 0.012 |
RMSD bond angle | 1.523 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.650 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.420 |
Number of reflections | 55770 | |
<I/σ(I)> | 14.4 | 2.8 |
Completeness [%] | 92.5 | 78.1 |
Redundancy | 4.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1M NA-CACODYLATE PH 6.0, 100MM NACL, 100UM ZNAC2, 20% PEG 4K |