2C2H
CRYSTAL STRUCTURE OF THE HUMAN RAC3 IN COMPLEX WITH GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-03 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.320, 110.320, 81.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.140 - 1.850 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g4u |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.773 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.070 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.800 |
| Rmerge | 0.070 | 0.510 |
| Number of reflections | 28734 | |
| <I/σ(I)> | 10.99 | 2 |
| Completeness [%] | 91.3 | 93.1 |
| Redundancy | 3.24 | 2.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% MPD, 0.5 M AMMONIUM SULFATE, 0.1 M HEPES PH 4.5 |
