2BXW

CRYSTAL STRUCTURE OF RHOGDI Lys(135,138,141)Tyr MUTANT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 31 2 1
Unit cell lengths	77.308, 77.308, 171.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.960 - 2.400
R-factor	0.18
Rwork	0.178
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kmt
RMSD bond length	0.008
RMSD bond angle	2.264
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.180
Number of reflections	23248
<I/σ(I)>	26.1	6.44
Completeness [%]	97.1	80.8
Redundancy	7.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.88		4M NA FORMATE, pH 7.88