2BWG
Structure of human guanosine monophosphate reductase GMPR1 in complex with GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-31 |
Detector | RIGAKU IMAGE PLATE |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.312, 114.418, 115.129 |
Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eep |
RMSD bond length | 0.013 |
RMSD bond angle | 1.352 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.120 | 0.370 |
Number of reflections | 154258 | |
<I/σ(I)> | 7.14 | 2 |
Completeness [%] | 90.1 | 80.8 |
Redundancy | 2.83 | 2.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 16% PEG3350 0.30 M K3CIT |