2BW0
Crystal Structure of the hydrolase domain of Human 10-Formyltetrahydrofolate 2 dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2005-06-04 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.226, 64.502, 58.891 |
Unit cell angles | 90.00, 98.18, 90.00 |
Refinement procedure
Resolution | 41.670 - 1.700 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s3i |
RMSD bond length | 0.016 |
RMSD bond angle | 1.621 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.700 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.090 | 0.380 |
Number of reflections | 167067 | |
<I/σ(I)> | 4.5 | 1.9 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1.7M AMMONIUM SULPLATE 50MM HEPES PH 7.8 |