2BUE
Structure of AAC(6')-Ib in complex with Ribostamycin and Coenzyme A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-18 |
| Detector | RIGAKU-MSC RAXIS-IV |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.475, 57.475, 148.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 149.070 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v0c |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.335 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.290 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.210 |
| Number of reflections | 27341 | |
| <I/σ(I)> | 33.2 | 6.9 |
| Completeness [%] | 97.0 | 84.7 |
| Redundancy | 7.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.75 | PROTEIN (10MG/ML, 20 MM TRIS PH 7.5, 20 MM NACL, 3 MM ACCOA) PRECIPITANT (20% PEG3350, 200 MM CALCIUM ACETATE, 100 MM NACACODYLATE PH 6.75) VAPOR DIFFUSION UNDER OIL |
