2BOI
1.1A Structure of Chromobacterium Violaceum Lectin CV2L in Complex with alpha-methyl-fucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.093, 89.836, 46.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.470 - 1.100 |
| R-factor | 0.102 |
| Rwork | 0.101 |
| R-free | 0.11700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.634 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.380 | 1.130 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 87549 | |
| <I/σ(I)> | 5.36 | 2.34 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.14 | 7.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 8000 10%, 0.1 M (NH4)2SO4 PH 5 |
