2BNU

Structural and kinetic basis for heightened immunogenicity of T cell vaccines

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-20
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	42.991, 60.295, 82.641
Unit cell angles	90.00, 89.54, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.245
Rwork	0.245
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oga
RMSD bond length	0.005
RMSD bond angle	1.310
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.460
High resolution limit [Å]	1.400	1.400
Rmerge	0.080	0.320
Number of reflections	85029
<I/σ(I)>	24.7	4.3
Completeness [%]	93.0	62.7
Redundancy	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8		PH 6.8