2BND

The structure of E. coli UMP kinase in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	140.434, 140.434, 59.993
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.500 - 2.600
R-factor	0.1948
Rwork	0.195
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bnf
RMSD bond length	0.007
RMSD bond angle	1.162
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.600	2.600
Rmerge	0.130	0.410
Number of reflections	12836
<I/σ(I)>	7.3	2.4
Completeness [%]	94.7	95.1
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.50