2BMB
X-ray structure of the bifunctional 6-hydroxymethyl-7,8- dihydroxypterin pyrophosphokinase dihydropteroate synthase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-18 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.566, 92.566, 192.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eye PDB ENTRY 1CBK |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.212 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.170 | 0.850 |
| Number of reflections | 37688 | |
| <I/σ(I)> | 22.4 | 3.8 |
| Completeness [%] | 99.1 | 91.1 |
| Redundancy | 10.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.17 M NAH2PO4, 0.63 M K2HPO4, PH 6.3 |
