2BHG
3C protease from type A10(61) foot-and-mouth disease virus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 141.589, 141.589, 43.677 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.760 - 1.900 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.24500 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 25640 | |
| <I/σ(I)> | 12.5 | 2.2 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE PAPER, pH 6.50 |
