2BH2
Crystal Structure of E. coli 5-methyluridine methyltransferase RumA in complex with ribosomal RNA substrate and S-adenosylhomocysteine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-19 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.061, 63.542, 112.019 |
| Unit cell angles | 90.00, 125.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwv |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.011 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.090 | 0.730 |
| Number of reflections | 59411 | |
| <I/σ(I)> | 12.7 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | RUMA-RNA-SAH COMPLEX WAS CRYSTALLIZED FROM 100 MM SODIUM CACODYLATE, PH 6.5, 1.5 M AMMONIUM SULFATE, AND 10 MM MGCL2 |
