2BCS
DNA polymerase lambda in complex with a DNA duplex containing an unpaired Dcmp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.968, 62.542, 140.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.900 - 2.200 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rzt |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | STRUCTURE (STUDIO) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 25896 | |
| Completeness [%] | 99.7 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Sodium citrate, sodium cacodylate, 2-propanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium citrate | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | 2-propanol | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium citrate | ||
| 6 | 1 | 2 | sodium cacodylate |
