2BCK
Crystal Structure of HLA-A*2402 Complexed with a telomerase peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.505, 132.186, 91.197 |
| Unit cell angles | 90.00, 128.80, 90.00 |
Refinement procedure
| Resolution | 78.570 - 2.800 |
| R-factor | 0.187 |
| Rwork | 0.183 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ao7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.348 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.620 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.153 | 0.917 |
| Number of reflections | 28450 | |
| <I/σ(I)> | 3.3 | 1.2 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.6M Ammonium Sulphate, 0.1M Sodium Chloride, 0.1M HEPES, 10% v/v Dioxane, pH 7.0, VAPOR DIFFUSION, temperature 293K |
