2BC4
Crystal structure of HLA-DM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9195 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.068, 108.420, 110.207 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.270 |
| R-factor | 0.248 |
| Rwork | 0.229 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HDM (Alanine substitution) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.413 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.370 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.057 | 0.309 |
| Number of reflections | 59797 | |
| <I/σ(I)> | 12.3 | 5.02 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 1.0M Lithium Sulfate, 0.3M Ammonium Sulfate, 0.1M Sodium Citrate Buffer, pH 5.4, vapor diffusion, hanging drop, temperature 277K |
