2BBQ

STRUCTURAL BASIS FOR RECOGNITION OF POLYGLUTAMYL FOLATES BY THYMIDYLATE SYNTHASE

Experimental procedure

Spacegroup name	P 63
Unit cell lengths	127.100, 127.100, 67.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 * - 2.300
R-factor	0.182
Rwork	0.182
RMSD bond length	0.015
RMSD bond angle	3.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.240 *
Rmerge	0.091 *
Total number of observations	62321 *
Number of reflections	26307 *
Completeness [%]	86.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution
2	1	reservoir	potassium phosphate	20 (mM)
3	1	reservoir	ammonium sulfate	2.3 (M)
4	1	reservoir	DTT	10 (mM)