2AZ1
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.280, 108.700, 119.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.22035 |
| Rwork | 0.217 |
| R-free | 0.28944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nue |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.776 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.800 |
| Number of reflections | 40514 | |
| <I/σ(I)> | 9.31 | |
| Completeness [%] | 99.1 | 97.2 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M CaCl2, 0.1M HEPES-Na, pH 7.5, 28% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
