2AYB

Crystal structure of HPV6a E2 DNA Binding Domain bound to a 16 base pair DNA target

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-20
Detector	BRUKER SMART 6000
Wavelength(s)	1.548
Spacegroup name	P 61
Unit cell lengths	75.247, 75.247, 97.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.230 - 3.200
R-factor	0.259
Rwork	0.189
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Protein of PDB entry 1R8H DNA of PDB entry 1JJ4
RMSD bond length	0.014
RMSD bond angle	2.148
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.230	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.138	0.387
Number of reflections	4928
<I/σ(I)>	19.8	5.2
Completeness [%]	99.4	100
Redundancy	22.5	22.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	tri-Sodium citrate dihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	tri-Sodium citrate dihydrate
2	1	1	H2O
3	1	2	tri-Sodium citrate dihydrate
4	1	2	H2O