2AWG
Structure of the PPIase domain of the Human FK506-binding protein 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-04 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.911, 53.795, 30.640 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 30.890 - 1.600 |
| R-factor | 0.16874 |
| Rwork | 0.167 |
| R-free | 0.20224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.890 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.049 | 0.183 |
| Number of reflections | 12477 | |
| <I/σ(I)> | 37.2 | 6.75 |
| Completeness [%] | 94.0 | 66.4 |
| Redundancy | 6.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 298 | 1.8M ammonium sulfate, 0.2M sodium acetate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 7.50 |
