2AO7

Adam10 Disintegrin and cysteine- rich domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-01
Detector	MARRESEARCH
Wavelength(s)	1.034, 0.9789, 1.016
Spacegroup name	I 41 3 2
Unit cell lengths	146.780, 146.780, 146.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
Rwork	0.261
R-free	0.28900
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	autoSHARP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000
High resolution limit [Å]	2.900
Rmerge	0.066
Number of reflections	11118
<I/σ(I)>	21.4
Completeness [%]	99.5
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	10 mM Hepes, 150 mM NaCl, 0.2M Ammonium Sulfate, 30% PEG 4000, soaked in 1 mM AuCl and 20% glycerol (cryoprotectant) , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K