2AMB
Crystal structure of human androgen receptor ligand binding domain in complex with tetrahydrogestrinone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-10 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.310, 66.040, 71.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.300 - 1.750 |
| R-factor | 0.194 |
| Rwork | 0.186 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ama |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.300 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.064 | 0.365 |
| Number of reflections | 25459 | 1358 |
| <I/σ(I)> | 18.01 | 3.01 |
| Completeness [%] | 94.6 | 63.8 |
| Redundancy | 6.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Na/K tartrate. MgSO4, Hepes, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
