2AL2
Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2005-04-04 |
Detector | BRUKER |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.700, 65.600, 85.900 |
Unit cell angles | 90.00, 99.20, 90.00 |
Refinement procedure
Resolution | 26.000 - 1.850 |
R-factor | 0.1961 |
Rwork | 0.196 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.240 |
Data reduction software | SADABS |
Data scaling software | SAINT |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.000 |
High resolution limit [Å] | 1.850 |
Number of reflections | 67650 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch | 8 | 293 | PEG 8000, potasssium chloride, pH 8.0, Batch, temperature 293K |