2AJ3
Crystal Structure of a Cross-Reactive HIV-1 Neutralizing CD4-Binding Site Antibody Fab m18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.599, 82.620, 187.204 |
Unit cell angles | 90.00, 95.27, 90.00 |
Refinement procedure
Resolution | 29.250 - 2.030 |
R-factor | 0.227 |
Rwork | 0.225 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hzh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.250 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.090 | 0.350 |
Number of reflections | 81882 | |
<I/σ(I)> | 11.6 | 1.89 |
Completeness [%] | 85.8 | 70 |
Redundancy | 3.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 292 | 100 mM CHES, 20% (w/v) PEG-8000, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |