2AHS
Crystal Structure of the Catalytic Domain of Human Tyrosine Receptor Phosphatase Beta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-04 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 123.368, 123.368, 179.569 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 53.400 - 2.100 |
R-factor | 0.155 |
Rwork | 0.152 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1RPM.PDB |
RMSD bond length | 0.013 |
RMSD bond angle | 1.337 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.400 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 47341 | |
Completeness [%] | 99.2 | 95.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 277 | PEG6000, Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 7.00 |