2AHE
Crystal structure of a soluble form of CLIC4. intercellular chloride ion channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.717, 79.478, 42.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.620 - 1.800 |
| R-factor | 0.19618 |
| Rwork | 0.194 |
| R-free | 0.22941 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1KOM |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.536 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.580 |
| Number of reflections | 25137 | |
| <I/σ(I)> | 8.3584 | 1.3 |
| Completeness [%] | 99.9 | 99.87 |
| Redundancy | 4.65 | 4.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
