2AG5
Crystal Structure of Human DHRS6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-14 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.092, 62.055, 74.042 |
| Unit cell angles | 106.05, 105.95, 100.97 |
Refinement procedure
| Resolution | 66.670 - 1.840 |
| R-factor | 0.16797 |
| Rwork | 0.167 |
| R-free | 0.22417 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.744 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.670 | 1.900 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 76176 | |
| Completeness [%] | 89.7 | 52.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | Ammonium sulphate, Mes, PEG monomethylether 5000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
